The chitosan/carboxymethyl cellulose/montmorillonite scaffolds incorporated with epigallocatechin-3-gallate-loaded chitosan microspheres for promoting osteogenesis of human umbilical cord-derived mesenchymal stem cell

Abstract Epigallocatechin-3-gallate (EGCG) is a plant-derived flavonoid compound with the ability to promote differentiation of human bone marrow-derived mesenchymal stem cells (MSCs) into osteoblasts. However, effect EGCG on osteogenic umbilical cord-derived (HUMSCs) rarely studied. Therefore, in this study, effects are studied HUMSCs by detecting cell proliferation, alkaline phosphatase (ALP) activity, calcium deposition and expression relevant markers. The results showed that can proliferation vitro at concentration 2.5–5.0 ?M. Unfortunately, easily metabolized during culture, which reduces its bioavailability. paper, EGCG-loaded microspheres (ECM) were prepared embedded chitosan/carboxymethyl cellulose/montmorillonite (CS/CMC/MMT) scaffolds form CS/CMC/MMT-ECM for improving bioavailability EGCG. cultured induce differentiation. scaffold continuously released up 22 days. In addition, osteoblast Taken together, present study suggested entrainment ECM CS/CMC/MMT was prospective scheme promotion HUMSCs. Graphical